Casein (Milk) Detection by Romer Labs AgraStrip


Background:  For the detection of bovine milk in foodstuffs, sensitive detection systems are required.  Approximately 80% of bovine milk proteins are caseins.  These heat-stable allergens represent the main fraction of bovine milk proteins.  Bovine milk is one of the most important allergenic food ingredients, especially for children.  Very low amounts of bovine milk can cause allergic reactions, which may lead to anaphylactic shock in severe cases.  Because of this, milk allergic persons must strictly avoid the consumption of milk or foods containing milk.  In particular, the risk of hidden milk proteins in foods such as sausage, cookies, ready meals or beverages represent a critical problem for milk allergic persons.

This analysis is intended to confirm the absence of Casein in food, rinse waters and swab samples.


Method

Apparatus

Turn Around Time

Sample Required – Product Samples

Sample Required – Rinse Water

Sample Required – Swab Samples

Type of Test

Reportable Units

Detection Limit – Product Samples

Detection Limit – Rinse Water

Detection Limit – Swab Samples

Romer Labs AgraStrip Casein

Romer Labs AgraStrip

Same Day for Swabs or 5-10 Business Days for Product

0.2 grams of a representative sample

0.5 mL Rinse Water

5cm x 5cm (25 cm2) or 10cm x 10 cm (100 cm2)

Lateral Flow Device; Test Strip

Product:  ppm = mg/L or Swabs:  µg/cm2

Lower Detection Limit:  1 ppm Casein

Lower Detection Limit:  0.06 ppm (60 ppb) Casein

Lower Detection Limit:  1 µg/25 cm2 or 0.25 µg/100 cm2 Casein


Analysis Description:  Immunological rapid test in lateral flow format for the detection of Casein in food, rinse waters and swab samples (environmental samples).  The extracted sample is transferred to an incubation vial that contains specific ready-to-use antibodies.  If the sample contains Casein, an antigen-antibody complex will form.  This is subsequently detected by the test strip.

Notes:  This assay is not appropriate for food products which are known to contain Casein.  If the sample contains more than 1% (10,000 ppm) Casein, the assay will be overwhelmed and give a false-negative result.

Sampling:  Consideration must be taken that the food may contain an uneven distribution of Casein (spot contamination).  It is important to provide a representative portion of food as only a small amount of material is tested.