Soy Detection by Romer Labs AgraStrip


Background:  Soybean (Glycine max) is a legume which has been used for thousands of years as a food. Soybean is highly nutritious and is composed of 39% protein, some of which are allergens, such as Gly m3, Gly m4, Glycinin and Trypsin-inhibitor.

Soy products are widely used in the food industry, namely as texturizers, emulsifiers and protein fillers. People allergic to soy must strictly avoid the consumption of soy containing-food products. Cross contamination during the production process often occurs and soy residues in food cannot be excluded, thus sensitive detection systems are required.

This analysis is intended to confirm the absence of Soy in food, rinse waters and swab samples.


Method

Apparatus

Turn Around Time

Sample Required – Product Samples

Sample Required – Rinse Water

Sample Required – Swab Samples

Type of Test

Reportable Units

Detection Limit – Product Samples

Detection Limit – Rinse Water

Detection Limit – Swab Samples

Romer Labs AgraStrip Soy

Romer Labs AgraStrip

Same Day for Swabs or 5-10 Business Days for Product

0.2 grams of a representative sample

0.5 mL Rinse Water

5cm x 5cm (25 cm2) or 10cm x 10 cm (100 cm2)

Lateral Flow Device; Test Strip

Product:  ppm = mg/L or Swabs:  µg/cm2

Lower Detection Limit:  2 ppm Soy

Lower Detection Limit:  0.11 ppm (110 ppb) Gluten

Lower Detection Limit:  2 µg/25 cm2 or 0.5 µg/100 cm2 Gluten


Analysis Description:  Immunological rapid test in lateral flow format for the detection of Soy in food, rinse waters and swab samples (environmental samples).  The extracted sample is transferred to an incubation vial that contains specific ready-to-use antibodies.  If the sample contains Soy, an antigen-antibody complex will form.  This is subsequently detected by the test strip.

Notes:  This assay is not appropriate for food products which are known to contain Soy.  If the sample contains more than 1% (10,000 ppm) Casein, the assay will be overwhelmed and give a false-negative result.

Sampling:  Consideration must be taken that the food may contain an uneven distribution of Soy(spot contamination).  It is important to provide a representative portion of food as only a small amount of material is tested.