Brazil Nut allergy is an increasing concern since allergies to tree nuts and seeds tend to be of an extremely severe nature, causing life-threatening and sometimes fatal reactions. Brazil nut, Bertholletia excelsa, which belongs to the order of Ericales, has a protein fraction of 13%. People allergic to Brazil nut must strictly avoid the consumption of food containing Brazil nut. Cross contamination during the production process often occurs and Brazil nut residues in food cannot be excluded. Therefore sensitive detection methods for Brazil nut in food are required.