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Residual Solvents (CDPHE Hemp)
Method Description

Samples are extracted into acetonitrile and cleaned with solid phase extraction. The extract is analyzed by LC-MS/MS and GC-MS/MS. Spiked recoveries are performed for each matrix.

Method

In-House Developed

Instrument

Headspace GC-MS

Turnaround Time

5 Business Days

Sample Required

5 g

Matrix

Botanicals, Extracts, Finished, Products, Raw Materials

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Residual Solvents (CDPHE Hemp)

Method Description

Samples are extracted into acetonitrile and cleaned with solid phase extraction. The extract is analyzed by LC-MS/MS and GC-MS/MS. Spiked recoveries are performed for each matrix.

Method

In-House Developed

Instrument

Headspace GC-MS

Turnaround Time

5 Business Days

Sample Required

5 g

Matrix

Botanicals, Extracts, Finished, Products, Raw Materials

Pass/Fail

See below for CDPHE Hemp Action Limits

Additional Information

CDPHE Hemp Product Limits per 6 CCR 1010-24
Solvents marked * are not approved for use. Due to their possible presence in the solvents approved for use, limits have been listed here accordingly.

1 Detected via LC-MS/MS

Full List of Analytes
&AnalyteUnitsLODLOQCDPHE Hemp Action Limit
Acetic Acid mg/kg (ppm) 10 50<1,000
Acetone mg/kg (ppm) 10 50<1,000
Anisole mg/kg (ppm) 10 50<1,000
Benzene* mg/kg (ppm) 0.5 2<2
n-Butane mg/kg (ppm) 2550<100
1-Butanol mg/kg (ppm) 200500<1,000
2-Butanol mg/kg (ppm) 10 20<1,000
Butyl Acetate mg/kg (ppm) 10 20<1,000
tert-Butylmethyl ether mg/kg (ppm) 10 20<1,000
Ethanol mg/kg (ppm) 30100<5,000
Ethyl Acetate mg/kg (ppm) 10 20<1,000
Ethyl Ether mg/kg (ppm) 10 20<1,000
Ethyl Formate mg/kg (ppm) 10 20<1,000
Formic Acid mg/kg (ppm) 10 50<1,000
n-Heptane mg/kg (ppm) 10 20<1,000
n-Hexane* mg/kg (ppm) 310<10
Isobutyl Acetate mg/kg (ppm) 10 20<1,000
Isopropyl Acetate mg/kg (ppm) 10 20<1,000
Methanol mg/kg (ppm) 10 50<100
Methyl Acetate mg/kg (ppm) 10 20<1,000
2-Methyl-1-Propanol (Isobutanol) mg/kg (ppm) 30100<1,000
Methylene Chloride mg/kg (ppm) 10 20<3
3-Methyl-1-Butanol mg/kg (ppm) 50150<1,000
Methylethyl Ketone (2-Butanone) mg/kg (ppm) 10 20<1,000
n-Pentane mg/kg (ppm) 10 50<1,000
1-Pentanol mg/kg (ppm) 50150<1,000
n-Propane mg/kg (ppm) 30100<100
1-Propanol mg/kg (ppm) 10 20<1,000
2-Propanol (Isopropyl Alcohol) mg/kg (ppm) 3001000<1,000
Propyl Acetate mg/kg (ppm) 10 20<1,000
Toluene* mg/kg (ppm) 10 20<20
Triethylamine1 mg/kg (ppm) <100
o-Xylene mg/kg (ppm) 10 20<40 Total
p,m-Xylenes mg/kg (ppm) 1040<40 Total
Acrylamide
Method Description

Samples are extracted and cleaned with QuEChERs. The extract is analyzed by LC-MS/MS.

Instrument

LC-MS/MS

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Acrylamide

Method Description

Samples are extracted and cleaned with QuEChERs. The extract is analyzed by LC-MS/MS.

Instrument

LC-MS/MS

Turnaround Time

5 Business Days

Sample Required

25 g

Matrix

Finished Products

Method

In-House Developed

Full List of Analytes
&AnalyteLOQLODUnits
Acrylamide0.0100.0025mg/kg
Aerobic Plate Count
Method Description

Representative sample portion is diluted with buffer, homogenized, plated on Petrifilm™, incub...

Method

AOAC OM 990.12 & AOAC OM 2015.13

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Aerobic Plate Count

Method Description

Representative sample portion is diluted with buffer, homogenized, plated on Petrifilm™, incubated at 35°C for 22-24 hours, after which the plates are interpreted and results reported.

Method

AOAC OM 990.12 & AOAC OM 2015.13

Instrument

Petrifilm®

Turnaround Time

1 Business Day

Sample Required

25 g or Contact Swab

Matrix

Food, Ingredient, Raw Meat, Hemp/CBD, Kratom, Nutraceuticals & Contact Swabs

Additional Information

Also known as Standard Plate Count (SPC) & Total Plate Count (TPC)

Full List of Analytes
&AnalyteLODLOQUnit
Aerobic Bacteria10 CFU10 CFUCFU/g or Contact Swab
Alcohols/Acetates
Method Description

Samples are extracted into water. The extract is analyzed by GC-FID.

Method

AOAC 984.14

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Alcohols/Acetates

Method Description

Samples are extracted into water. The extract is analyzed by GC-FID.

Method

AOAC 984.14

Instrument

GC-FIC

Turnaround Time

5 Business Days

Sample Required

25 g

Matrix

Hand Sanitizer, Drinks

Full List of Analytes
&AnalyteLOQLODUnits
Ethanol0.0100.0025%
Methanol0.0100.0025%
Ash
Method Description

Samples are combusted at > 500° C. The residue mass is compared to the sample mass to calcu...

Method

AOAC 923.03

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Ash

Method Description

Samples are combusted at > 500° C. The residue mass is compared to the sample mass to calculate ash.

Method

AOAC 923.03

Instrument

Muffle (Gravimetric)

Turnaround Time

5 Business Days

Sample Required

25 g

Matrix

Food, Ingredients, Botanicals

Full List of Analytes
&AnalyteLOQLODUnits
Ash0.1000.025%
Aspergillus
Method Description

Representative sample portion is homogenized with highly selective enrichment media and incuba...

Method

AOAC PTM 022103 AspergillusPRO™

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Aspergillus

Method Description

Representative sample portion is homogenized with highly selective enrichment media and incubated at 37°C for 48 hours, after which DNA is extracted and analyzed for the target pathogen.

Method

AOAC PTM 022103 AspergillusPRO™

Instrument

GENE-UP® PCR

Turnaround Time

5 Business Days

Sample Required

10 g

Matrix

Hemp/CBD, Food & Ingredients

Pass/Fail Limits

Undetected

Full List of Analytes
&AnalyteLODLOQUnit
Aspergillus flavus1 viable cell1 viable cellSample Required
Aspergillus fumigatus1 viable cell1 viable cellSample Required
Aspergillus niger1 viable cell1 viable cellSample Required
Aspergillus terrerus1 viable cell1 viable cellSample Required
B. cereus Enumeration
Method Description

Representative sample portion is diluted with buffer, homogenized, plated on selective agar, i...

Method

Bacara Agar

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B. cereus Enumeration

Method Description

Representative sample portion is diluted with buffer, homogenized, plated on selective agar, incubated at 30°C for 24 hours, after which the plates are interpreted and results reported.

Method

Bacara Agar

Instrument

Agar

Turnaround Time

2 Business Days

Sample Required

25 g

Matrix

Food, Ingredient & Raw Meat

Full List of Analytes
&AnalyteLODLOQUnit
Bacillus cereus10 CFU10 CFUCFU/g
Caffeine
Method Description

Samples are extracted into boiling water. The extract is analyzed by HPLC-UV.

Method

AOAC 980.14

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Caffeine

Method Description

Samples are extracted into boiling water. The extract is analyzed by HPLC-UV.

Method

AOAC 980.14

Instrument

HPLC-UV

Turnaround Time

5 Business Days

Sample Required

25 g

Matrix

Drinks, Powder, Raw Material

Full List of Analytes
&AnalyteLOQLODUnits
Caffeine0.2500.100mg/g
Calories
Method Description

Samples are analyzed for proximates and calories are calculated by the 4-4-9 method. Additiona...

Instrument

Calculation

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Calories

Method Description

Samples are analyzed for proximates and calories are calculated by the 4-4-9 method. Additional considerations must be taken if the product contains alcohols/sugar alcohols or significant dietary fiber.

Instrument

Calculation

Turnaround Time

5 Business Days

Sample Required

25 g

Matrix

Food, Ingredients, Botanicals

Method

USDA Agriculture Handbook No. 74

Full List of Analytes
&AnalyteRange, Low EndRange, High EndUnits
Calories0NA/100g
Cannabinoid incl. THC Potency
Method Description

Samples are extracted into methanol or isopropanol. The extract is analyzed by HPLC-UV.

Method

In-House Developed

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Cannabinoid incl. THC Potency

Method Description

Samples are extracted into methanol or isopropanol. The extract is analyzed by HPLC-UV.

Method

In-House Developed

Instrument

HPLC-UV

Turnaround Time

5 Business Days

Sample Required

25 g

Matrix

Botanicals, Extracts, Finished Products

Full List of Analytes
&AnalyteUnitsLODLOQ
Cannabichromene (CBC)mg/g, %0.010.2
Cannabicitran (CBT)mg/g, %0.010.2
Cannabidiol (CBD)mg/g, %0.010.1
Cannabidiolic Acid (CBDA)mg/g, %0.010.1
Cannabidivarin (CBDV)mg/g, %0.010.1
Cannabigerol (CBG)mg/g, %0.010.1
Cannabinol (CBN)mg/g, %0.010.1
Delta - 9 THC (THC)mg/g, %0.010.1
Delta 9 - Tetrahydrocannabinolic Acid (THCA-A)mg/g, %0.010.1
Potential CBDmg/g, %0.010.1
Potential THCmg/g, %0.010.1
Tetrahydrocannabivarin (THCV)mg/g, %0.010.1
Carbohydrates
Method Description

Samples are analyzed for proximates and carbohydrates are calculated by subtraction. Additiona...

Instrument

Calculation

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Carbohydrates

Method Description

Samples are analyzed for proximates and carbohydrates are calculated by subtraction. Additional considerations must be taken if the product contains alcohols/sugar alcohols or significant dietary fiber.

Instrument

Calculation

Turnaround Time

5 Business Days

Sample Required

25 g

Matrix

Food, Ingredients, Botanicals

Method

FAO Food and Nutrition Paper 77

Full List of Analytes
&AnalyteLOQLODUnits
Total Carbohydrates, Crude1.00N/A%
Cholesterol
Method Description

Samples are saponified prior to extraction/concentration of cholesterol. The organic extract i...

Method

AOAC 994.10

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Cholesterol

Method Description

Samples are saponified prior to extraction/concentration of cholesterol. The organic extract is subjected to derivatization and analyzed by gas chromatography.

Method

AOAC 994.10

Instrument

GC-FID

Turnaround Time

5 Business Days

Sample Required

25 g

Matrix

Food, Ingredients, Botanicals

Full List of Analytes
&AnalyteLOQLODUnits
Cholesterol2.000.050mg/100g
Coconut
Method Description

This lateral flow test an an immunochromatographic rapid test for the qualitative detection of...

Method

Gold Standard Diagnostics SENSIStrip Coconut

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Coconut

Method Description

This lateral flow test an an immunochromatographic rapid test for the qualitative detection of the specified allergen in foodstuffs, rinse waters, and Contact Swab. Samples are extracted, the extract is transfed to a vial containing allergen specific antibodies. A test-strip inserted into the vial and detects any allergen-antibody reactions by a color change.

Method

Gold Standard Diagnostics SENSIStrip Coconut

Instrument

Lateral Flow

Turnaround Time

3 Business Days

Sample Required

5 g, Contact Swab or Rinsate

Matrix

Food, Ingredient, Rinsate & Contact Swabs

Pass/Fail Limits

Undetected

Additional Information

Reported as Present or Absent

Rapid allegen testing assays are for screening purposes, so a positive result might require confirmation or further testing.

Full List of Analytes
&AnalyteLODLOQUnits
Coconut55ppm
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